This document is an instruction manual for the Lambda ZAP Premade Library, intended for in vitro use only. The product is covered by a limited warranty, which only includes replacement of the product. Agilent does not provide any other warranties, express or implied, including but not limited to implied warranties of merchantability or fitness for a particular purpose. Agilent shall not be liable for any direct, indirect, consequential, or incidental damages arising out of the use, the results of use, or the inability to use this product.
The document is an instruction manual for the Agilent Lambda ZAP Premade Library. It details the materials provided, such as amplified premade library constructs in the Lambda ZAP vector, host strains (XL1-Blue MRF' and BB4), and interference-resistant helper phages (R408 and VCSM13). The manual specifies that all components must be stored at -80°C and should not pass through more than two freeze-thaw cycles.
The guide covers the complete workflow for using the library, starting with establishing bacterial stocks and determining background via color selection. It includes detailed protocols for titering helper phages and the library itself, performing plaque lifts to isolate colonies, and hybridizing probes to screen for specific inserts. Additionally, it explains how to excise the pBluescript phagemid from the Lambda ZAP vector in vivo using single-clone or mass excision methods.
| DNA insert length range | <1 to 10 kb |
|---|---|
| Library storage temperature | -80°C |
| Maximum freeze-thaw cycles | two |
The XL1-Blue MRF' strain is used for plating excised phagemids.
The BB4 strain is used for all other manipulations.
The kit includes R408 and VCSM13 interference-resistant helper phages.