Lambda FIX II Library INSTRUCTION MANUAL BN #936001-11 Revision A For In Vitro Use Only
This document provides instructions for using the Lambda FIX II Library, a premade amplified library constructed in the Lambda FIX II vector. The kit includes the library, XL1-Blue MRA host strains, and necessary glycerol stocks for cloning large genomic DNA fragments.
The manual covers essential laboratory procedures including preparing host strains (XL1-Blue MRA and XL1-Blue MRA P2), creating bacterial glycerol stocks, plating cultures, amplifying the library over three days, performing plaque lifts, hybridizing and screening clones, and rapid restriction mapping. It also includes recipes for media preparation such as LB Agar, NZY Broth, and various buffers.
| Library Volume Provided | 1 ml |
|---|---|
| Host Strain Stock Volume | 0.5-ml bacterial glycerol stock |
| Library Storage Temperature | -80°C |
| Host Strain Storage Temperature | -80°C |
Store the library at -80°C immediately upon arrival.
Do not pass through more than two freeze-thaw cycles.
Store at -80°C, though they may be stored at -20°C.