Lambda EMBL3/BamH I Vector Kit instruction manual, including product features, usage, warranty information and ordering information
This document provides instructions for using the Lambda EMBL3/BamH I Vector Kit (Catalog #241211) and the related Gigapack III Gold Cloning Kit (#241612). The kit is designed for in vitro use to create genomic libraries by cloning BamH I-compatible DNA fragments ranging from 9 to 23 kb into a lambda phage vector system.
| Insert Size Range | 9 to 23 kb |
|---|---|
| Vector Digestion Sites | EcoR I and BamH I |
| Compatible Insert Enzymes | Sau3A I, Mbo I, Bgl II, or BamH I |
| Vector Concentration | 1 μg/μl |
| Expected Recombinant Plaques | 1 × 10^6–1.5 × 10^7 |
No, do not CIAP-treat the insert DNA because the provided Lambda EMBL3 vector arms have already been treated.
The ligation reaction should be incubated at 4°C overnight.