Lambda ZAP-CMV XR Predigested Vector Kit INSTRUCTION MANUAL is a product manual from Agilent. It mainly introduces the use and precautions of the product.
This document provides instructions for using the Lambda ZAP-CMV XR Predigested Vector Kit (Catalog #239222) for in vitro eukaryotic expression. The kit includes a vector digested with Xho I and EcoR I, bacterial host strains (XL1-Blue MRF' and XLOLR), and interference-resistant helper phages (ExAssist and R408). It covers storage conditions, preparation of bacterial stocks, and titering procedures.
| Vector Digestion Sites | Xho I and EcoR I |
|---|---|
| Vector Quantity | 10 ug |
| Test Insert Quantity | 2.5 ug |
| Vector Capacity | up to 6.5 kb |
| ExAssist Helper Phage Titer | ~1.0 x 10^9 pfu/ml |
| R408 Helper Phage Titer | ~7.5 < 10^7 pfu/ml |
| Vector Storage Temperature | -20°C |
Use the XLOLR strain for plating excised phagemids.
The R408 interference-resistant helper phage is recommended for single-stranded rescue.
Store the Helper Phage at -80°C.