Lambda FIX II Undigested Vector Kit instruction manual
The document serves as an instruction manual for the Lambda FIX II Undigested Vector Kit (Catalog #248201), a replacement vector system designed for cloning large fragments of genomic DNA. The kit includes undigested Lambda FIX II DNA and host strains such as XL1-Blue MRA and XL1-Blue MRA (P2). It details the selection mechanism based on spi (sensitive to P2 inhibition) and provides protocols for preparing bacterial glycerol stocks.
| Vector Quantity | 10 pg |
|---|---|
| Storage Temperature (Long-term) | -20°C |
| Short-term Storage Duration | 1 month |
The kit provides XL1-Blue MRA strain and XL1-Blue MRA (P2) strain as bacterial glycerol stocks.
The vector should be stored at -20°C immediately after arrival.