Agilent Technologies Lambda CE6 Induction Kit Instruction Manual

Lambda CE6 Induction Kit INSTRUCTION MANUAL


Agilent Technologies Lambda CE6 Induction Kit Instruction Manual - cover page
Brand
Lambda
Document type
User Manual
Language
English
Pages
16
File format
PDF
File size
137 KB
Published
06 June, 2012
Updated
01 October, 2023
MD5 checksum
B1A8507A921D7E7272AD0FD23AB8F22E

About this manual

The Lambda CE6 Induction Kit is designed to deliver T7 RNA polymerase into BL21 competent cells, enabling the tight control of toxic protein expression from T7 promoter-driven vectors. The kit includes bacteriophage lambda CE6, LE392 host cells, and BL21 competent cells.

What's inside

  • Materials Provided
  • Storage Conditions
  • Academic and Nonprofit Laboratory Assurance Letter
  • Host and Expression Strains
  • Amplifying the Lambda CE6 Bacteriophage
  • Titering the Lambda CE6 Bacteriophage
  • Transformation Guidelines
  • Induction of Target Protein by Infection

Specifications

Bacteriophage lambda CE6 titer25.0 x 10^9 pfu/ml
Storage temperature for all materials-80°C

Frequently asked questions

What is the purpose of the Lambda CE6 Induction Kit?

The kit is used to deliver T7 RNA polymerase into BL21 competent cells to induce expression of toxic proteins from T7 promoter-driven vectors.

What are the expected titers for the lambda CE6 bacteriophage?

Expect titers of approximately 5 x 10^9 pfu/ml.

What additional tubes are required for the procedure?

You need 14-ml BD Falcon polypropylene round-bottom tubes.

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