Lambda DASH II Undigested Vector Kit instruction manual
The document serves as an instruction manual for the Lambda DASH II Undigested Vector Kit (Catalog #247201), a replacement vector system designed for cloning large fragments of genomic DNA. The kit includes undigested Lambda DASH II DNA and two host strains: XL1-Blue MRA and XL1-Blue MRA (P2). The manual details the selection mechanism based on P2 phage lysogeny, where recombinant phages can grow on the P2 strain while non-recombinants cannot.
| Host strain XL1-Blue MRA stock volume | 0.5-ml bacterial glycerol stock |
|---|---|
| Host strain XL1-Blue MRA (P2) stock volume | 0.5-ml bacterial glycerol stock |
| Vector storage temperature (long-term) | -20°C |
| Bacterial Glycerol Stock storage temperature | -80°C |
It serves as a control strain and allows for the selection of recombinant phages because Lambda DASH II DNA containing active red and gam genes cannot grow on it.
After thawing, it should be aliquoted and stored at -20°C. For very short-term storage, it can be kept at 4°C for up to one month.
The protocol recommends using 2-5 U of enzyme per ug of DNA.