Lambda ZAP-CMV RI Predigested Vector Kit Instruction Manual
This document provides instructions for the Lambda ZAP-CMV RI Predigested Vector Kit (Catalog #239221), a system designed to allow eukaryotic expression of DNA inserts up to 6.5 kb in length. The kit includes pre-digested vector, bacterial host strains (XL1-Blue MRF' and XLOLR), and interference-resistant helper phages.
| Insert size limit | 6.5 kb |
|---|---|
| Vector titer (ExAssist) | ~1.0 x 10^10 pfu/ml |
| Vector titer (R408) | ~7.5 < 10^7 pfu/ml |
| Vector storage temperature | -20°C |
The XLOLR strain is recommended for plating excised phagemids.
The XL1-Blue MRF' strain is recommended for all other manipulations.
The Lambda ZAP-CMV RI vector accommodates DNA inserts up to 6.5 kb in length.