This document is an instruction manual for the Lambda DASH II/BamH I Vector Kit, detailing the usage and features of the product.
The Lambda DASH II system is a replacement vector used for cloning large fragments of genomic DNA. It utilizes spi (sensitive to P2 inhibition) selection to allow only recombinant phages to grow on specific host strains like XL1-Blue MRA (P2). The kit includes the double-digested vector, test inserts, host strains, and packaging extracts.
| Vector Quantity (Catalog #247211) | 10 pg |
|---|---|
| Test Insert Quantity (Catalog #247211) | 2.5 ug |
| Host Strain Stock Volume | 0.5-ml bacterial glycerol stock |
| Packaging Extract Quantity (Catalog #247613) | 11 x 25 ul |
| Control DNA Quantity | 1.05 wg |
The vector should be stored at -20°C.
The Lambda DASH II vector must be stored at -20°C after thawing and aliquoting.
Host strains can be stored at 4°C for up to one month.
Packaging extracts must not be allowed to thaw under any circumstances.