All-in-OneTM Q-PCR Primer Array User's Manual is a product manual published by GeneCopoeia Inc. It mainly introduces the use of the product, precautions, product form and other information
The All-in-OneTM Q‑PCR Primer Array is a ready‑to‑use 96‑well plate containing 96 optimized primer pairs, including four internal reference genes. It enables rapid, quantitative gene‑expression profiling using Real‑Time PCR with SYBR Green detection.
The manual provides product overview, storage and handling precautions, detailed experimental procedures (RNA preparation, cDNA synthesis, qPCR setup, and data analysis), instrument compatibility, and troubleshooting tips. An appendix describes the ΔΔCt data‑analysis method and includes a specific Wnt‑signaling panel example. This manual is written in Chinese.
| Number of primer pairs | 96 |
|---|---|
| Internal reference genes | 4 primer pairs |
| Storage temperature | +2 °C ~ +8 °C |
| Ready‑to‑Use format | Add Q‑PCR Mix, cDNA and water only |
| Instrument compatibility | Four packaging formats for major Real‑Time PCR instruments |
Over 95 % of the primers are designed across introns, which prevents amplification of genomic DNA, so a separate contamination control is unnecessary.
Efficiency can be inferred from the Ct values of internal reference genes (e.g., GAPDH or ACTB); a Ct > 25 indicates possible problems.
The RNA OD260/280 ratio should be between 1.7 and 2.0.
A 28S : 18S ratio of approximately 2 : 1 is considered acceptable.