Key facts
- Brand
- GeneCopoeia
- Model
- C0210A
- Category
- Other
- Industry
- Health
- Documents
- 2
- Languages
- ZH
- Last added
- 2000-01-01
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GeneCopoeia C0210A First Strand cDNA Synthesis Kit User Manual
The First Strand cDNA Synthesis Kit (product code C0210A) from GeneCopoeia uses Moloney Murine Leukemia Virus reverse transcriptase (M‑MLV RTase, RNase H‑) to synthesize single‑strand cDNA from total RNA or poly(A) mRNA. All necessary reagents, including 5× RT Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, and Sequence‑Specific Primer, are supplied in a single kit.
The manual outlines storage conditions, handling precautions, and a complete workflow: preparation of the RNA‑Primer mix, reverse transcription (37 °C or 42 °C for 1 hour depending on primer), enzyme inactivation at 85 °C, and downstream PCR or real‑time PCR usage. An experimental example demonstrates how to verify reverse‑transcription efficiency by PCR amplification of GAPDH. This manual is written in Chinese.
GeneCopoeia C0210A specifications
| Storage temperature | -20°C |
|---|---|
| Reaction total volume | 13 µl |
| Enzyme | M‑MLV Reverse Transcriptase (RNase H‑) |
| 5× RT Reaction Buffer | Included |
| RNase Inhibitor concentration | 25 U/µl |
Source: GeneCopoeia C0210A First Strand cDNA Synthesis Kit User Manual
All GeneCopoeia C0210A documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
At what temperature should the kit be stored?
The kit should be stored at –20 °C.
From: GeneCopoeia C0210A First Strand cDNA Synthesis Kit User Manual
How long and at what temperature should the reverse transcription be performed with Oligo(dT)18?
Incubate at 42 °C for 1 hour.
From: GeneCopoeia C0210A First Strand cDNA Synthesis Kit User Manual
How should the kit be stored?
The kit should be stored at –20 °C.
From: GeneCopoeia First Strand cDNA Synthesis Kit C0210A User Manual
What incubation temperature and time are required for reverse transcription with Oligo(dT)18 or Sequence‑Specific Primer?
Incubate at 42 °C for 1 hour.
From: GeneCopoeia First Strand cDNA Synthesis Kit C0210A User Manual