This paper introduces a method for the separation of basic drugs, such as ranitidine and paroxetine, based on hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization mass spectrometry (MS/MS) detection. The method showed good linearity over the 0.5–100 ppb levels. A spiked serum was cleaned up by protein precipitation and an aliquot was directly injected into the HILIC/ESI-MS system. Recovery was found to be 98% for ranitidine and 79% for paroxetine at the 100 ppb level.
The document describes a method for separating two basic drugs, ranitidine and paroxetine, from human serum. The study compares the separation efficiency of Reversed-Phase Liquid Chromatography (RPLC) against Hydrophilic Interaction Chromatography (HILIC). Results indicate that HILIC provides improved peak shape for ranitidine and faster analysis times compared to RPLC under the tested conditions.
Recovery was found to be 98% for ranitidine and 79% for paroxetine at the 100 ppb level.
The method utilized a ZORBAX RX-SIL Silica column with dimensions of 2.1 mm x 150 mm.