This Application Note describes the general use of cell fluorescence assays on the Agilent 2100 bioanalyzer. The cell assay extension together with the cell fluorescence LabChip® kit allows both the analysis of pre-stained cells and the analysis of cells by on-chip staining. Cell assays on the 2100 bioanalyzer are based on simple flow cytometric analysis. The technical principles, as well as the typical workflow in sample and chip preparation are described here. A typical dye optimization experiment was performed and the appropriate dye concentration for a specific cell line determined. The cell fluorescence software is easy-to-use and allows different forms of data evaluation and result presentation. Using an example, data evaluation is explained in detail. Specific advantages of cell fluorescence analysis with the Agilent 2100 bioanalyzer are low cell and reagent consumption, ease-of-use, automated data acquisition and evaluation of up to six samples.
The document details how to perform cell fluorescence analysis using the Agilent 2100 bioanalyzer with the cell assay extension and LabChip kit. It explains that the system uses simple flow cytometric analysis based on a two-color detection system (red laser at 635 nm and blue LED at 470 nm) to measure fluorescent labeled cells.
A cell concentration of 2 million/ml is recommended to achieve a statistically relevant number of events.
The kit allows for the analysis of six samples per chip.
The default sample run time is 4 minutes, which typically yields 500 to 1000 measured events.