This technical note describes some of the typical quality control tests and specifications for Agilent 2100 Bioanalyzer Protein 200 Plus LabChip Kits.
This document provides a technical overview of the Agilent Protein 200 Plus LabChip kits used for microfluidic protein analysis. It describes how the technology integrates sample handling, separation, staining, destaining, detection, and digital data processing to achieve high throughput and reproducibility.
The text details specific quality control tests performed on the kits, including sizing accuracy, resolution, detection limits, linear dynamic range, and area reproducibility. Results from various instruments and buffer conditions demonstrate that the kits meet factory specifications for protein analysis.
| Sizing range | 14-200 kDa |
|---|---|
| Typical sizing accuracy | 20% C.V. |
| Sizing resolution | 10% difference in MW |
| Minute detectable concentration (PBS) | 20 pg/mL BSA |
| Minute detectable concentration (0.5 M NaCl) | 40 pg/mL BSA |
| Samples per chip | 10 + ladder |
The results are typically within 20% C.V. of the expected value.
The assay can detect 20 pg/mL of BSA in PBS with a good signal-to-noise ratio.
The linear dynamic range is specified for 0.02—2 mg/mL of protein.