Using DMSO as an Injection Solvent to Increase Sample Load in Preparative LC Application Note

About this manual

In preparative high-performance liquid chromatography (HPLC), increasing the injection size to maximize sample load can lead to unexpected solubility problems, such as peak broadening or splitting. This document addresses these issues by demonstrating that using dimethylsulfoxide (DMSO) as the injection solvent improves sample solubility without causing interference with the analytes of interest.

What's inside

• Abstract
• Introduction
• Results
• Using DMSO to Increase Sample Solubility and Sample Load
• DMSO as an Injection Solvent
• Conclusions

Frequently asked questions

What is the benefit of using DMSO as an injection solvent?

Using DMSO increases sample solubility and sample load while avoiding peak splitting.

Which Agilent columns are compatible with DMSO injection?

DMSO elutes at or near the void volume on ZORBAX Bonus-RP, Eclipse XDB, StableBond, and Extend HPLC Columns.