The Stratagene QPCR Human Reference Total RNA instruction manual provides usage, storage, assay optimization, standardization, and troubleshooting guidance.
The QPCR Human Reference Total RNA is a high‑quality control material composed of total RNA from ten human cell lines, designed for quantitative PCR gene‑expression analysis. It is supplied as 25 µg of RNA and stored at –80 °C to maintain integrity.
The manual details the materials provided, required equipment, storage conditions, assay‑optimization protocols for SYBR® Green, TaqMan® and molecular beacon assays, standard‑curve generation, and troubleshooting tips. It also includes technical specifications, recommended primer and probe concentrations, and instrument settings for Stratagene’s Mx series and ABI PRISM platforms.
| Quantity supplied | 25 µg (1 µg/µl in 0.1 mM EDTA/RNase‑free H₂O) |
|---|---|
| Storage temperature | -80 °C |
| Catalog number | 750500 |
Store all components at –80 °C.
The kit supplies 25 µg of RNA (1 µg/µl in 0.1 mM EDTA/RNase‑free water).
The protocols are compatible with Stratagene’s Mx3000P, Mx3005P, Mx4000, and ABI PRISM 7700 instruments.
Stratagene recommends titrating each primer between 50 and 300 nM.