This manual provides instructions for the Promega Lumit IL-4 (Human) Immunoassay, a homogeneous bioluminescent kit for detecting IL-4 in cell culture samples.
The Lumit IL-4 (Human) Immunoassay is a homogeneous, bioluminescent assay designed to detect interleukin-4 (IL-4) released from cells without the need for sample transfers or wash steps. Based on NanoLuc Binary Technology (NanoBiT), the system uses a pair of anti-human IL-4 monoclonal antibodies covalently labeled with Small BiT (SmBiT) and Large BiT (LgBiT). When these labeled antibodies bind to IL-4, the complementary subunits are brought into proximity to reconstitute the NanoBiT enzyme, generating a luminescent signal proportional to the analyte amount. This manual is written in Chinese.
| Total Assay Time | 70 minutes |
|---|---|
| Catalog Number W6060 | 100 assays |
| Catalog Number W6061 | 1,000 assays |
The complete assay takes 70 minutes.
The Lumit™ Immunoassay protocol is complete in 70 minutes.
The W6060 kit includes 3.2ml of Lumit™ Detection Buffer B.