Promega cAMP-Glo Detection Assay Quick Reference Guide V1502/V1503

This document provides a quick reference guide for the Promega cAMP-Glo™ detection assay (catalog numbers V1502 and V1503), detailing components, storage…

Brand: Promega
Document type: User Manual
Language: Chinese
Pages: 4
File format: PDF
File size: 198 KB
Published: 21 October, 2026
Updated: 21 October, 2026
MD5 checksum: E3B2361727CC44641E9796BF3BE912F4
PDF: Promega cAMP-Glo Detection Assay Quick Reference Guide V1502/V1503 PDF

About this manual

This document serves as a concise operational guide for the Promega cAMP-Glo™ Detection Assay, specifically for catalog numbers V1502 (3,000 tests) and V1503 (30,000 tests). It outlines the kit components, storage conditions at -20°C, and preparation instructions for reagents such as Kinase-Glo® Buffer and cAMP solutions. The guide is designed to support experiments in 384-well plates. This manual is written in Chinese.

What's inside

Product Components
Storage Conditions
Cell Preparation
Reagent Preparation
Standard Curve Creation
Detection Steps
EC50 Determination
IC50 Confirmation
Z' Factor Calculation

Specifications

Assay Format	384-well plate
V1502 Capacity	3,000 tests
V1503 Capacity	30,000 tests

Frequently asked questions

Which cell types are supported by this assay?

The assay is suitable for adherent cells, suspension cells, and frozen cells.

What volume of cAMP-Glo™ Lysis Buffer is added per well for a 384-well plate?

Add 7.5µl of cAMP-Glo™ Lysis Buffer to all wells.

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