The Promega TNT® T7 Quick for PCR DNA kit manual provides step‑by‑step instructions, component details, storage conditions, and troubleshooting for in‑vitro…
The TNT® T7 Quick for PCR DNA kit enables rapid, coupled transcription‑translation of PCR‑generated DNA templates. The kit contains a master mix and reagents that allow direct use of PCR products without purification, producing high protein yields.
The manual covers template considerations, component list and storage, general lab practices, detailed transcription/translation procedures (including radioactive, non‑radioactive, and fluorescent detection options), post‑translation analysis, and a FAQ section for common problems. This manual is written in Chinese.
| Master Mix storage temperature | -70 °C |
|---|---|
| Typical reaction volume | ≈40 × 50 µl |
| Master Mix composition | 1.6 ml TNT® T7 PCR Quick Master Mix (8 × 200 µl) |
| Methionine supplement | 50 µl, 1 mM |
| Incubation temperature for transcription/translation | 30 °C |
Incubate the reaction at 30 °C for 60–90 minutes.