This manual provides operating instructions for the Promega Eastep Minipreps DNA Purification Kit (LS1010/LS1012) for isolating plasmid DNA from E. coli.
The Eastep Minipreps DNA Purification Kit is designed for the rapid and reliable isolation of plasmid DNA from Escherichia coli. The kit is effective for plasmids smaller than 20,000 bp and allows for purification from 1-10 ml of overnight E. coli culture within approximately 30 minutes. The purified DNA is suitable for downstream applications such as DNA fluorescent automated sequencing and in vitro transcription. The kit includes reagents for alkaline protease digestion, cell lysis, neutralization, and purification columns, with optional vacuum manifolds available separately. This manual is written in Chinese.
| Effective Plasmid Size | Plasmids smaller than 20,000 bp |
|---|---|
| Sample Volume Range | 1-10 ml overnight culture |
| Processing Time | Approximately 30 minutes |
| Storage Temperature | 15-30°C |
| Vacuum Manifold Requirement | Not included (Catalog No. A1331) |
Do not exceed 5 minutes to prevent nicking of plasmid DNA.
Yes, DNA in TE buffer is stable at 4°C, but TE should not be added if used for automated fluorescent sequencing.
The vacuum pressure must reach at least 15 inches of mercury.