This document provides concise operating instructions for the Promega TNT Quick Coupled Transcription/Translation System kits (L1170, L1171, L5620, L5610…
This document serves as a concise instruction guide for the Promega TNT Quick Coupled Transcription/Translation System, covering multiple kit models including L1170, L1171, L5620, L5610, and L5070. It outlines the necessary materials and reagents required for the experiment, such as template vectors, specific kit components, and equipment for protein analysis.
The guide details the step-by-step procedures for preparing transcription/translation templates, conducting the coupled reaction, analyzing translation products via protein gel electrophoresis, performing Western blotting, and detecting results through colorimetric or luciferase assays. It also includes specific protocols for positive control detection using the Luciferase Assay Reagent. This manual is written in Chinese.
The system can express proteins up to 210 kD, though this requires optimized reaction conditions.
The reaction mixture should be incubated at 30°C for 60 to 90 minutes.
The membrane should be incubated in TBST at room temperature for 60 minutes.