This manual describes the Promega CTB323 Caspase-Glo® 3/7 Assay, a homogeneous luminescent system for detecting caspase-3 and -7 activities in cell lysates.
The Caspase-Glo® 3/7 Assay is a homogeneous, luminescent assay designed to measure caspase-3 and caspase-7 activities. The reagent contains a luminogenic substrate with the DEVD sequence optimized for caspase activity, luciferase activity, and cell lysis. Upon adding the single reagent in an 'add-mix-measure' format, cells are lysed, followed by caspase cleavage of the substrate to generate a stable 'glow-type' luminescent signal produced by Ultra-Glo™ Recombinant Luciferase. This manual is written in Chinese.
The assay uses an 'add-mix-measure' format where a single reagent is added to samples.
Caspase and luciferase enzyme activities reach steady state, peaking in approximately one hour.