Technical manual for the Promega Bright-Glo Luciferase Assay System (E2610, E2620, E2650) used to quantify luciferase expression in mammalian cells.
The Bright-Glo™ Luciferase Assay System is designed for ultrahigh-throughput quantitation of luciferase expression in mammalian cells. It provides robust, homogeneous assay chemistry with high sensitivity and a signal half-life of approximately half an hour without prior sample processing. The system includes Bright-Glo™ Luciferase Assay Buffer and Substrate to form the reagent.
| Product Size E2610 capacity | perform 100 assays of 100µl each |
|---|---|
| Product Size E2620 capacity | perform 1,000 assays of 100µl each |
| Product Size E2650 capacity | perform 10,000 assays of 100µl each |
| Substrate storage temperature | -20°C or 4°C for up to two weeks |
| Buffer storage temperature | below 25°C |
The system is designed for use with RPMI 1640, MEM α, DMEM and Ham's F12 containing 0–10% serum.
Wait at least 2 minutes to allow complete cell lysis before measuring in a luminometer.
It shows 10% loss per 5 hours at room temperature, 10% loss per 24 hours at 4°C, and <5% loss after one month at –70°C.