Bio‑Rad Bio‑Plex Pro Human Cytokine Assays User Manual

Instruction manual for the Bio‑Plex Pro Human Cytokine Assays kit, covering assay principle, workflow, reagents, and troubleshooting.


Bio‑Rad Bio‑Plex Pro Human Cytokine Assays User Manual - cover page
Brand
BIO-RAD
Category
Other
Document type
User Manual
Language
English
Pages
26
File format
PDF
File size
589 KB
Published
05 October, 2026
Updated
05 October, 2026
MD5 checksum
6D90FF4B79F1AFCB12C4B701B9EBF20C

About this manual

The Bio‑Plex Pro Human Cytokine Assays kit enables quantification of a panel of 48 cytokines, chemokines, and growth factors in serum, plasma, culture supernatant and other sample types. The manual describes the multiplex bead‑based immunoassay principle, required reagents, recommended materials, and detailed step‑by‑step workflow.

It also provides guidance on instrument setup (Bio‑Plex 200, 3D or MAGPIX readers), data acquisition using Bio‑Plex Manager™ or Luminex xPONENT software, safety considerations, troubleshooting tips, and ordering information for reagents and accessories.

What's inside

  • Description
  • Principle
  • Kit Contents and Storage
  • Recommended Materials
  • Assay Workflow
  • Important Considerations
  • Detailed Instructions
  • Troubleshooting Guide
  • Safety Considerations
  • Ordering Information

Specifications

Number of analytes48 cytokines, chemokines and growth factors
Sample volume per well12.5 µl
Storage temperature4 °C, never frozen
Shelf life after purchaseminimum 6 months

Frequently asked questions

How many cytokines are measured by the Bio‑Plex Pro Human Cytokine Assays?

The kit quantifies a panel of 48 cytokines, chemokines and growth factors.

What sample volume is required per well?

Each well uses 12.5 µl of sample.

How should the kit be stored?

Store the kit at 4 °C and never freeze the reagents.

Which software can be used to read the assay data?

Data can be acquired with Bio‑Plex Manager™ Software or Luminex xPONENT Software.

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