Model 491 Prep Cell Instruction Manual, introduces the use of the product, maintenance and other contents.
This manual describes the Model 491 Prep Cell, a preparative electrophoresis system for purifying proteins or nucleic acids. It covers general information, accessory equipment, specifications, chemical compatibility, and safety guidelines. Detailed sections explain the major components, assembly steps, operation procedures, and how to optimize running conditions for SDS‑PAGE.
Additional chapters address disassembly and cleaning, troubleshooting, preparation of electrophoresis buffers, gel preparation, native‑PAGE methods, and product information including part numbers and accessories. Contact information for technical service and warranty terms are also provided.
| Upper buffer chamber material | acrylic |
|---|---|
| Lower buffer chamber material | acrylic |
| Electrode material | platinum, 0.010 inch diameter |
| Lid material | acrylic |
| Gel tube assembly material | glass/acrylic |
| Elution chamber base material | acrylic |
| Elution frit material | polyethylene |
| Support frit material | polyethylene |
| Cooling core material | glazed alumina |
| Elution tube material | borosilicate glass, 0.06" ID |
| Shipping weight | 6 lb |
| Overall size | 7 in. diameter x 14 in. high |
| Voltage limit | 500 volts |
| Current limit | 40 milliamperes |
| Power limit | 20 watts |
| Cooling buffer flow rate | 100 ml/min |
| Elution buffer flow rate | 60 ml/hour |
| Upper electrophoresis buffer volume | 300‑600 ml |
| Elution buffer chamber volume | 900 ml |
| Lower electrophoresis buffer volume | 2‑3 liter |
The Model 491 Prep Cell is warranted against defects in materials and workmanship for one year from the date of purchase.
The recommended power supply is Bio‑Rad’s Model 1000/500 Power Supply, though the PowerPac 3000 can also be used.
The maximum operating voltage is 500 VDC.