This manual provides instructions for assembling, operating, and maintaining the Whole Gel Eluter and Mini Whole Gel Eluter.
The Whole Gel Eluter and Mini Whole Gel Eluter are preparative electrophoresis instruments for recovering proteins from SDS or native PAGE slab gels. The manual describes the instrument’s construction, safety features, and recommended accessories such as filter paper, cellophane membranes, and vacuum harvesting components.
Key sections cover specifications, detailed component descriptions, step‑by‑step assembly, operating procedures, troubleshooting, and cleaning/sterilization. Safety interlocks, power‑supply requirements, and recommended operating limits are highlighted to ensure safe laboratory use.
| Construction material (base) | acrylic/polycarbonate insert |
|---|---|
| Construction material (elution chamber core) | acrylic/polycarbonate insert |
| Voltage limit | 300 V (Whole Gel), 200 V (Mini) |
| Power limit | 15 W (Whole Gel), 10 W (Mini) |
| Elution sample volume | ~3 ml (Whole Gel), ~0.5 ml (Mini) |
| Overall size | 5.0" H × 6.0" W × 6.0" D (Whole Gel), 5.0" H × 10.5" W × 10.5" D (Mini) |
The Whole Gel Eluter is limited to 300 VDC, while the Mini Whole Gel Eluter is limited to 200 VDC.
The lid assembly provides a safety interlock that breaks current flow when the lid is removed.
The recommended power supply is the Bio‑Rad PowerPac 300.
The Whole Gel Eluter can operate up to 15 W, and the Mini version up to 10 W.