Promega PepTag Nonradioactive PKC or cAMP-Dependent Protein Kinase Assay System User Manual

PepTag® nonradioactive protein kinase C or cAMP-dependent protein kinase assay system provides a rapid, sensitive, nonradioactive method for detecting protein kinase C (PKC) and cAMP-dependent protein kinase (PKA).


Promega PepTag Nonradioactive PKC or cAMP-Dependent Protein Kinase Assay System User Manual - cover page
Brand
Promega
Category
Other
Document type
User Manual
Language
Chinese
Pages
21
File format
PDF
File size
732 KB
Published
16 October, 2023
Updated
16 October, 2023
MD5 checksum
D27DE3FD1DE4DA1F8D1160C5F3977BA2

About this manual

The PepTag Nonradioactive Protein Kinase C or cAMP-Dependent Protein Kinase Assay System (Catalog No. CTB132) offers a rapid, sensitive, and nonradioactive method for detecting Protein Kinase C (PKC) and cAMP-dependent Protein Kinase (PKA) activity. The system utilizes fluorescent short peptide substrates that change net charge upon phosphorylation, allowing for the separation of phosphorylated and non-phosphorylated peptides via agarose gel electrophoresis. This manual is written in Chinese.

What's inside

  • Description
  • Product Components and Storage
  • Qualitative Assay Steps
  • Sample Preparation for PKC
  • PKC Assay Steps
  • Sample Preparation for PKA
  • PKA Assay Steps
  • Gel Preparation
  • Electrophoresis
  • Quantitative Analysis
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