This document describes the application of the ENLITEN® ATP bioluminescence assay kit produced by Yuanpinghao Biotechnology Co., Ltd. on the ModulusTM microplate multifunctional detector. The assay kit can be used for rapid and sensitive detection of the content of bacteria, yeast, fungi, and other microorganisms, or for the detection of the amount of ATP generated or consumed in enzyme-catalyzed reactions.
The document describes how to use the Promega ENLITENe ATP Bioluminescence Assay Kit with the Turner Modulus TM Microplate Multifunctional Detector. This combination provides a rapid and sensitive method for detecting bacteria, yeast, fungi, and other microbes, or measuring ATP generation/consumption in enzymatic reactions. The kit allows detection of attomole levels of ATP. This manual is written in Chinese.
The kit provides a dynamic range greater than 6 orders of magnitude.
The reaction should ideally be performed at a temperature of 23-25C and a pH of 7.7.