Key facts
- Brand
- GeneCopoeia
- Model
- C0201A
- Documents
- 2
- Languages
- ZH · JA
- Last added
- 2012-03-15
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GeneCopoeia C0201A M-MLV Reverse Transcriptase (RNase H-) User Manual
This manual provides an overview of the GeneCopoeia C0201A M‑MLV Reverse Transcriptase (RNase H‑) kit, including product description, enzyme activity, purity, and recommended applications such as cDNA library construction and RT‑PCR. It also lists the available package sizes (50 µl, 250 µl, 1 ml) and storage conditions.
The document outlines the experimental workflow for first‑strand cDNA synthesis, storage guidelines, reaction component mixing, and temperature steps. A FAQ section answers common questions about the advantages of RNase H‑deficient enzyme and template RNA choices. This manual is written in Chinese.
GeneCopoeia C0201A specifications
| Enzyme activity | 200 U/µl |
|---|---|
| Storage temperature | -20 °C |
| Storage buffer composition | 150 mM NaCl, 50 mM Tris‑HCl (pH 7.6), 0.1 mM EDTA, 1 mM DTT, 0.1 % NP‑40, glycerol |
| Reaction buffer (5× RT) | 25 mM each dNTP, 150 mM NaCl, 50 mM Tris‑HCl (pH 7.6), 0.1 mM EDTA, 1 mM DTT, 0.1 % NP‑40, glycerol |
Source: GeneCopoeia C0201A M-MLV Reverse Transcriptase User Manual
All GeneCopoeia C0201A documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
Can total RNA be used as the template for the RT reaction, or is mRNA required?
Both total RNA and mRNA can be used; the success of the RT reaction depends on RNA purity, not the RNA type.
From: GeneCopoeia C0201A M-MLV Reverse Transcriptase (RNase H-) User Manual