This document introduces the application of Agilent MIXED gel technology in gel permeation chromatography and size exclusion chromatography columns. This technology allows different pore size gels to be blended together to create a column with a linear calibration suitable for a specific molecular weight range. Selectivity in GPC/SEC separations can be controlled by adjusting the column length and the pore size distribution of the packing material. Compared to individual pore size gels, MIXED gel packing provides a larger molecular weight range and better separation performance.
This document provides a technical overview of Agilent's MIXED gel technology used in gel permeation chromatography (GPC) and size exclusion chromatography (SEC). It explains how blending individual pore size gels creates columns with linear calibration curves suitable for specific molecular weight ranges, offering superior resolution compared to single-pore-size gels over limited ranges.
The text details the selection criteria for Agilent PLgel columns based on application needs. It compares MIXED gel packing (e.g., PLgel 10 µm MIXED-B) with individual pore size gels (e.g., PLgel 5 µm 104A), highlighting their respective strengths in separating high molecular weight additives versus discrete, narrow polydispersity polymers.
| Column Length (Example) | 7.5 x 300 mm |
|---|---|
| Flow Rate (Test Conditions) | 1.0 mL/min |
| Eluent (Test Conditions) | THF (stabilized) |
| Detector Wavelength | 254 nm |
MIXED gel columns exhibit a linear calibration spanning a molecular weight range suitable for particular applications, whereas individual pore size gels provide resolution only over a limited range with a relatively shallow slope.
The MIXED gel approach, particularly PLgel 10 µm MIXED-B, is generally most favorable for high molecular weight additives.
The linear resolving range for this packing is from 200 to 2,000,000.