This article introduces the method of combining a mass spectrometer (MS) with a high-performance liquid chromatography (HPLC-UV, DAD) system, and extending the capabilities of HPLC-UV by connecting a mass spectrometer. The article discusses the development and advantages of LC-MS systems, as well as considerations for sample preparation methods.
This document serves as a technical overview for integrating mass spectrometry (MS) into existing high-performance liquid chromatography (HPLC) systems. It explains how coupling MS to an HPLC-UV system enhances analytical capabilities by providing specificity, sensitivity, and structural elucidation of unknown compounds.
The three typical atmospheric pressure ionization methods are ESI (Electrospray Ionization), APCI (Atmospheric Pressure Chemical Ionization), and APPI (Atmospheric Pressure Photoionization).
Non-volatile buffers can be replaced by volatile buffers at the same pH to interface well with the mass spectrometer.
Adding MS provides specificity unattainable by any other detection scheme and allows for peak identification and elucidation of unknown structures.