This document provides an overview of preparative gel permeation chromatography, which improves the resolution of sample components by recycling the sample back through the column multiple times before fraction collection, thus conveniently minimizing cross-fraction contamination. The high column volume of the Agilent PLgel 10 µm 100 Å, 25 × 300 mm prep column facilitates recycle capability. Improved resolution after three cycles is observed.
This document describes a method to improve resolution in preparative gel permeation chromatography by recycling the sample back through the column multiple times before fraction collection. This technique minimizes cross-fraction contamination and is particularly effective with high column volume Agilent PLgel columns.
| Column | 2.x Agilent PLgel 10 um 100A, 25 x 300 mm |
|---|---|
| Eluent | THF |
| Flow rate | 9.0 mL/min |
| Detector | VWD, 254 nm |
| System | Agilent 1260 Infinity GPC-SEC Analysis System |
The high column volume of the Agilent PLgel 10 um 100A, 25 x 300 mm prep column facilitates recycle capability.
The specified flow rate for the eluent THF is 9.0 mL/min.
The improved resolution after three cycles is apparent in the provided figure.