Agilent PLgel 5 µm Effect of Injection Volume on Oligomer Separation Technical Overview

This white paper describes the effect of different injection volumes on the separation of oligomers using an Agilent PLgel 5µm column

Brand: Agilent
Category: Other Electronics
Document type: Product Leaflet
Language: English
Pages: 2
File format: PDF
File size: 94 KB
Published: 05 July, 2012
Updated: 30 September, 2023
Model: PLgel 5 µm
MD5 checksum: 1A2FD00666BC16274352922A45B9E6BC
PDF: Agilent PLgel 5 µm Effect of Injection Volume on Oligomer Separation Technical Overview PDF

About this manual

This technical overview investigates the impact of injection volume on the resolution of oligomers during gel permeation chromatography (GPC). It demonstrates that overloading can severely decrease resolution by comparing results from two different injection volumes on an Agilent PLgel 5 µm column.

What's inside

Introduction
Effect of Injection Volume
Conditions
Calibrants
Columns
Eluent
Flow rate
Detector
System
Agilent Literature Library

Specifications

Column Dimensions	300 x 7.5 mm
Part Number	PL1110-6520
Eluent	THF
Flow Rate	1.0 mL/min
Concentration	0.1%
System	Agilent PL-GPC 50

Frequently asked questions

Which column was used to demonstrate the effect of different injection volumes?

An Agilent PLgel 5 µm column was used for the demonstration.

What is the specified flow rate for the separation conditions?

The flow rate is set to 1.0 mL/min.

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