Describes the importance of loading in preparative gel permeation chromatography (GPC) and how loading can be significantly increased for very low molecular weight materials such as epoxy resins.
This document provides a technical overview of loading parameters for preparative gel permeation chromatography (GPC). It explains that loading capacity depends on molecular weight and should generally scale up at least 10 times from analytical to preparative GPC, with potential for significant increases for very low molecular weight materials like epoxy resins.
| Column | 2x Agilent PLgel 10 um 100A, 25 x 300 mm |
|---|---|
| Eluent | THF |
| Flow rate | 9.0 mL/min |
| Analytical Loading | 5 mg/mL, 1 mL (5 mg on-column) |
| Preparative Loading | 100 mg/mL, 3 mL (300 mg on-column) |
| Detector | RI |
| System | Agilent 1260 Infinity GPC-SEC Analysis System |
There should be at least 10 times scale up from analytical to preparative GPC.
Yes, for very low molecular weight materials such as epoxy resins, the loading can be significantly increased.