This white paper introduces the oligomer separation effect of Agilent PLgel 5 µm columns at different flow rates
This document provides a technical overview of the effect of flow rate on oligomer separation in gel permeation chromatography (GPC). It illustrates that resolution improves as the flow rate is reduced, although this results in increased run time. The demonstration utilizes an Agilent PLgel 5 µm column with three different flow rates.
| Column Type | Agilent PLgel 5 µm |
|---|---|
| Column Part Number | PL1110-6520 |
| Column Dimensions | 300 x 7.5 mm |
| Eluent | THF |
| System | Agilent PL-GPC 50 |
| Calibrant | Agilent Polystyrene Standard 480 |
The resolution of oligomers improves as the flow rate is reduced.
The run time is obviously increased as a result of reducing the flow rate.