Agilent High Sensitivity DNA Kit Technical Note for 2100 Bioanalyzer

This document introduces the Agilent High Sensitivity DNA kit, which can be used for sizing, quantification and quality control of DNA fragments. The kit's sensitivity is up to pg/µL, and it can detect DNA fragments in the range of 50-7000bp.


Agilent High Sensitivity DNA Kit Technical Note for 2100 Bioanalyzer - cover page
Brand
Agilent
Document type
Product Leaflet
Language
English
Pages
8
File format
PDF
File size
1.4 MB
Published
05 July, 2012
Updated
30 September, 2023
MD5 checksum
5C0ADA9015A848566A4FE2EB1DAA0ACF

About this manual

This document is a technical note describing the performance of the Agilent High Sensitivity DNA kit when used with the Agilent 2100 Bioanalyzer. The kit is designed for high-sensitivity on-chip DNA sizing and quantification, extending the product portfolio to cover double-stranded DNA fragments and complex samples like sequencing libraries in a size range of 50-7000 bp.

What's inside

  • Introduction
  • Overview
  • Materials and equipment
  • DNA smear analysis
  • Sizing range
  • Sizing accuracy and reproducibility
  • Sensitivity
  • Linear dynamic range

Specifications

Sizing range50-7000 bp
Analysis run time45 minutes
Samples per chip1
Kit size10 chips

Frequently asked questions

What is the sizing accuracy of the High Sensitivity DNA kit?

The specified sizing accuracy is +10% CV.

What is the sizing reproducibility of the kit?

The specified sizing reproducibility is 5% CV.

What is the maximum salt concentration allowed in the sample?

The maximum salt concentration is 10 mM Tris and 1 mM EDTA.

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