This note illustrates the effect of particle size and injection volume on the efficiency of Agilent PLgel 100Å, 300 × 7.5 mm columns.
The text discusses two factors affecting column efficiency and resolution: the particle size of packing materials and system dead volume. Smaller particles lead to higher efficiencies, but minimizing extra-column dispersion is necessary for optimal performance with small particle sizes.
It illustrates these concepts using Agilent PLgel 3 pm, 5 pm, and 10 pm 100A columns tested with polystyrene 580 samples. The document demonstrates that decreasing injection volume from 100 pL to 20 pL significantly improves resolution.
The document specifies that the Agilent PLgel 100A columns have dimensions of 300 x 7.5 mm.
The chromatograms were obtained using a flow rate of 1.0 mL/min.
The effective MW range for this column extends up to 4,000.