This Application Note describes how to optimize a typical SFC separation by changing: • System backpressure • Modifi er concentration • Column temperature
This document serves as a technical overview for optimizing Supercritical Fluid Chromatography (SFC) separations using the Agilent 1260 Infinity Analytical SFC System. It investigates the influence of system backpressure, organic modifier concentration, and column temperature on the selectivity and resolution of a four-compound mixture.
The text provides experimental results demonstrating how increasing backpressure decreases retention times but can affect resolution differently for various peak pairs. It also analyzes the impact of methanol concentration on separation efficiency and shows that lower column temperatures (10 °C) can improve resolution for specific analyte mixtures compared to higher temperatures (40 °C). Finally, it offers general recommendations on pre-conditioning mobile phase temperatures to minimize baseline noise in the detector.
| System Model | Agilent 1260 Infinity Analytical SFC System |
|---|---|
| Flow Rate | 3 mL/min |
| Column Temperature Range | 10 °C to 40 °C |
| Backpressure Range | 110 to 190 bar |
| Publication Date | July 1, 2011 |
The document investigates the influence of system backpressure, column temperature, and modifier concentration to fine-tune selectivity.
Increasing the system backpressure results in decreased retention times because the density of the supercritical fluid is changed.