MassCode PCR technology is suitable for any research application that requires detection of a set of nucleic acid sequences through design of consensus primers, and is ideal for high-throughput detection of 10-30 target sequences simultaneously. MassCode PCR technology leverages Agilent’s reliable high-performance LC/MS instrumentation and expertise in polymerase enzyme engineering and PCR reagent development to provide a novel amplification and detection solution.
MassCode PCR is a multiplex polymerase chain reaction technology designed for the simultaneous detection of a set of nucleic acid sequences, ideal for high-throughput applications requiring the analysis of 10 to 30 target sequences. The technology combines Agilent's high-performance LC/MS instrumentation with expertise in polymerase enzyme engineering and PCR reagent development.
| Target detection range | 10-30 target sequences |
|---|---|
| Plate format | 96-well plate |
| Detection method | LC/MS with APCI source |
It is suitable for research applications requiring the detection of a set of nucleic acid sequences through the design of consensus primers.
The workflow starts with Reverse Transcription PCR (RT-PCR) on the source material in a 96-well plate format after nucleic acid extraction.