This document provides a technical overview of calibrating Agilent PLgel 3 µm 100Å columns using polystyrene standards for GPC analysis. Agilent PLgel 3 µm 100Å columns are designed for the analysis of low molecular weight discrete molecules in organic solvents. The molecular weight information of a sample is calculated by analyzing a series of narrow distribution polymers of known molecular weight and generating a standard calibration curve. This example demonstrates the typical calibration data using polystyrene narrow standards in tetrahydrofuran.
This document provides a technical overview for calibrating Agilent PLgel 3 µm 100A columns, which are designed for analyzing low molecular weight discrete molecules in organic solvents. The calibration process utilizes a series of polystyrene narrow standards with known molecular weights to generate a calibration curve.
| Column dimensions | 300 x 7.5 mm |
|---|---|
| Concentration | 0.1% (w/v) |
| Flow rate | 1.0 mL/min |
| Injection volume | 20 pL |
| Exclusion limit | 4,000 g/mol |
| Efficiency | greater than 100,000 plates per meter |
It is designed specifically for the analysis of low molecular weight discrete molecules in organic solvents.
The calibration is linear up to the exclusion limit of 4,000 g/mol.
The column has a guaranteed efficiency greater than 100,000 plates per meter.