DNA Marker product introduction
Stratagene offers a collection of DNA markers designed to serve as ideal standards for gel electrophoresis, covering a wide range of fragment sizes from 8 bp to 23 kb. These markers feature known mass amounts in each band, allowing for easy relative mass estimation of DNA fragments of similar size when visualized with intercalating dyes like ethidium bromide.
The portfolio includes various specific markers such as the Kb DNA Ladder, Supercoiled DNA Ladder, and Lambda/Hind III Marker. All products are double-stranded DNA provided in a 10 mM Tris-HCl (pH 7.5) and 1 mM EDTA solution. They are noted for high ladder purity ensuring sharp bands and are easily radio-labeled, making them perfect for Northern or Southern blot analysis.
| Fragment Size Range | 8 bp to 23 kb |
|---|---|
| Solution Buffer | 10 mM Tris-HCL (pH 7.5), 1 mM EDTA |
| DNA Type | Double stranded DNA |
| Kb DNA Ladder Fragments | 15 fragments: 250 bp to 12 kb |
| Supercoiled DNA Ladder Fragments | 10 fragments: 2 kb to 12 kb |
| Lambda/Hind III Marker Fragments | 8 fragments: 125 bp to 23 kb |
| Illuminator™ Kb DNA Ladder Fragments | 15 fragments: 250 bp to 12 kb |
The collection of DNA markers serves as ideal gel electrophoresis standards from 8 bp to 23 kb.
Yes, they are easily radio-labeled and perfect for Northern or Southern blot analysis.
All ladders are provided in 10 mM Tris-HCL (pH 7.5), 1 mM EDTA solution.