This document introduces the method of separating basic compounds using Agilent ZORBAX Stablebond column, including column conditions, pH effect on separation selectivity.
This document presents chromatographic data for the separation of six substituted aniline compounds: p-anisidine, m-toluidine, 3-amino-benzonitrile, p-chloroaniline, m-chloroaniline, and o-chloroaniline. The analysis was performed using a ZORBAX SB-C18 column with a mobile phase consisting of 42% methanol and 58% 25 mM phosphate buffer at pH 2.5.
Basic compounds separate with good peak shape on ZORBAX Stablebond columns.
Selectivity is very dependent on pH of mobile phase and control of pH is critical to stability of separation.