This document describes the separation of a series of substituted anilines.
The application note details a chromatographic method for separating various substituted aniline compounds. The analysis was performed on an Agilent HP-1050 system using a ZORBAX SB-C8 column with dimensions of 4.6 x 75 mm.
Key highlights include achieving isocratic separation in just two minutes while maintaining excellent peak efficiencies and shapes at a flow rate of 3.0 mL/min. The method operates at a temperature of 60°C, resulting in modest back pressure.
Socratic separation is achieved in 2 minutes using a column packed with 3.5um ZORBAX SB-C8.
The procedure requires a flow rate of 3.0 mL/min at a temperature of 60°C.