This document describes how to use high-performance liquid chromatography (HPSEC) to monitor differences in synthetic polymer production. Specifically, the material uses the ZORBAX Bimodal S column (Agilent P/N: 880957-814) to perform size exclusion separations and uses ultraviolet detection (UV 254 nm) to detect the quality of polymethacrylate materials.
This document highlights the use of High Performance Size Exclusion Chromatography (HPSEC) to monitor differences in synthetic polymer production. It specifically demonstrates how polymethacrylate material with good properties shows clear size exclusion separation differences from batches with poor properties.
The application utilizes specific chromatographic conditions including a temperature of 35°C, UV detection at 254 nm, and THF as the mobile phase. The analysis employs ZORBAX Bimodal S columns (Agilent P/N: 880957-814) with a flow rate of 1.0 mL/min.
The separation uses a temperature of 35°, UV detection at 254 nm, THF as the mobile phase, a ZORBAX Bimodal S column, and a flow rate of 1.0 mL/min.
The application uses a ZORBAX Bimodal S column with a flow rate of 1.0 mL/min.