Agilent Urea Analysis Application Note

This document introduces a method for analyzing highly hydrophilic compounds using the Agilent ZORBAX Rx-SIL column and a reversed-phase mobile phase. This method can effectively separate urea and uracil, which are both unretained by reversed-phase columns because they are highly hydrophilic.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 207 KB
Published: 03 July, 2012
Updated: 01 October, 2023
MD5 checksum: B3CA5A0C4E66F1E9A57095A6CE44E5C1
PDF: Agilent Urea Analysis Application Note PDF

About this manual

Organic Chemicals Robert Ricker explains that amines, urea, and uracil can be difficult to analyze by standard reversed-phase HPLC due to excessive retention or lack of retention. The document presents a solution using the Agilent ZORBAX Rx-SIL column with a specific mobile phase to achieve rugged chromatography for these hydrophilic compounds.

What's inside

Application
Operating Conditions
Highlights

Frequently asked questions

What column is used for the urea analysis described in this note?

The analysis uses a ZORBAX Rx-SIL column with dimensions of 4.6 x 150 mm.

What is the flow rate for the HPLC system in this procedure?

The flow rate is set to 1 mL/ min.

What detection wavelength is required for this urea analysis?

Detection is performed at UV 210 nm.

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