This document describes the working principle and features of the Agilent 1100 Series purification system. The system allows for fractionation and re-analysis of compounds using the same instrument without any hardware modification. The software enables automated re-analysis of fractions, allowing for automated and unattended operation of the fractionation/re-analysis process. The document also provides details on the specific operation methods and conditions for fractionation and analysis using the system.
The document describes a workflow for purifying compounds using the Agilent 1100 Series purification system. It details how the system performs initial fractionation on a preparative column followed by automated re-analysis of collected fractions on an analytical column without hardware modification.
The process utilizes specific equipment including two preparative pumps, a thermostatted column compartment with a switching valve, and a diode array detector. The note provides experimental conditions such as flow rates, column dimensions, and mobile phases used to demonstrate the system's ability to perform unattended, automated purification.
No, the system allows both fractionation and re-analysis to be done with the same instrument without any hardware modification.
Yes, the software allows for automated re-analysis of fractions, meaning the whole process can be performed automatically and unattended.