This document describes the method and advantages of compound purification using the Agilent 1100 Series purification system. The use of a mass-selective detector enables mass-based fraction collection, enhancing and simplifying time- and peak-based fraction collection methods.
This document describes an application of preparative HPLC using the Agilent 1100 Series purification system connected to a mass-selective detector (MSD). The primary goal is to show how mass-based fraction collection simplifies compound identification compared to traditional time- or peak-based methods.
The text details three specific strategies for collecting fractions: time-based, peak-based, and mass-based. It compares the number of collected fractions and sample recovery rates for each method, concluding that while mass-based collection reduces fractions to a single vial per run, it results in significant sample loss compared to other methods.
Recovery locations are assigned to a specific sample to collect everything that is not collected as a fraction, preventing the loss of parts of the injected sample.
The time-based fraction collection strategy resulted in the collection of 80 fractions.
The flow rate for the time-based fraction collection method was set to 25 mL/min.