Agilent 1100 Effect of Mobile Phase Preparation on Chromatography Application Note

This document shows the effect of different mobile phase preparation methods on retention behavior in an Agilent 1100 LC system.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 681 KB
Published: 03 July, 2012
Updated: 30 September, 2023
MD5 checksum: 1E561D276C389B1F7D63D23D5A914250
PDF: Agilent 1100 Effect of Mobile Phase Preparation on Chromatography Application Note PDF

About this manual

This document investigates the impact of mobile phase preparation methods on chromatography results using an Agilent 1100 HPLC system with a ZORBAX Eclipse XDB-C8 column. It compares four specific preparation techniques: Dial-a-Mix, Volume/Volume mixing, Mix-to-Mark, and Weight/Weight mixing.

What's inside

Application
Operating Conditions
Highlights
Mobile Phase Preparation Methods
Elution Pattern Changes

Specifications

HPLC System	Agilent 1100 with quatemary pump
Column	ZORBAX Eclipse XDB-C8 Rapid-Resolution (3.5m), 4.6 x 50 mm
Detection	UV 254 nm
Flow Rate	1 mL/ min
Temperature	ambient

Frequently asked questions

Which mobile phase preparation method is more accurate?

Weight/Weight (w/w) is more accurate than volume/volume because weight is independent of temperature.

What is the purpose of Uracil in this experiment?

Uracil is unretained and serves to indicate proper flow rate.

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