This document describes the role of insulin in the human body and presents the results of a quality control experiment on insulin.
The document explains that aqueous SEC is an important tool for analyzing genetically engineered or pig insulin. It illustrates how improper storage conditions lead to degradation, characterized by additional peaks in the chromatogram and a disappearance of the main insulin peak compared to good samples stored under adequate conditions.
The note details the specific experimental conditions used for this analysis, including sample preparation at 20 °C, a PSS Suprema 100 column, and a phosphate buffer mobile phase. It also lists the components of the Agilent 1100 Series GPC-SEC system employed to perform these analyses.
Insulin has a molecular weight of approximately 6000.