This document introduces HPLC methods for the analysis of two antihypertensive drugs
The document details an HPLC method for separating two antihypertensive drugs, captopril and enalapril. The analysis utilizes gradient elution on a Zorbax SB-C18 reversed-phase column with UV detection at 204 nm.
Experimental conditions include the use of an Agilent 1100 Series HPLC system, specific mobile phases (KH2PO4 in water and acetonitrile), and a temperature of 60°C. The note highlights the performance data, including limits of detection and precision.
| HPLC System | Agilent 1100 Series HPLC |
|---|---|
| Detector Wavelength | 204 nm |
| Column Type | Zorbax SB-C18, 3.5 µm, 4.6 x 75 mm |
| Injection Volume | 5 µL |
| Column Temperature | 60 °C |
| Flow Rate | 1.0 mL/min |
The method analyzes captopril and enalapril.
The LOD for Captopril is 0.5 mg/L.